製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Human
    • 配列
      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN
    • 分子量
      27 kDa
    • 領域
      1 to 200
    • タグ
      His tag N-Terminus
    • 配列の追加情報
      UBE2K produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-200a.a.) and having a molecular mass of 26.5kDa.

特性

Our Abpromise guarantee covers the use of ab73665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.
    ab73665 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • 製品の状態
    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 1mM DTT, 1X PBS, pH 7.5

  • 再構成
    Reconstitute in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution ab73665 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

関連情報

  • 別名
    • E2 25K
    • E2(25K)
    • HIP-2
    • Huntingtin interacting protein 2
    • Huntingtin-interacting protein 2
    • HYPG
    • LIG
    • UBC1
    • UBE2K
    • UBE2K_HUMAN
    • Ubiquitin carrier protein
    • Ubiquitin conjugating enzyme E2 25 kDa
    • ubiquitin conjugating enzyme E2K
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast)
    • Ubiquitin protein ligase
    • Ubiquitin-conjugating enzyme E2 K
    • Ubiquitin-conjugating enzyme E2(25K)
    • Ubiquitin-conjugating enzyme E2-25 kDa
    • Ubiquitin-conjugating enzyme E2-25K
    • Ubiquitin-protein ligase
    see all
  • 機能
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • 組織特異性
    Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • 翻訳後修飾
    Sumoylation at Lys-14 impairs catalytic activity.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt

参考文献

ab73665 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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