製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN
    • 分子量27 kDa
    • 領域1 to 200
    • タグHis tag N-Terminus
    • 配列の追加情報UBE2K produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-200a.a.) and having a molecular mass of 26.5kDa.

特性

Our Abpromise guarantee covers the use of ab73665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    ab73665 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 1mM DTT, 1X PBS, pH 7.5

  • 再構成Reconstitute in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution ab73665 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

関連情報

  • 別名
    • E2 25K
    • E2(25K)
    • HIP-2
    • Huntingtin interacting protein 2
    • Huntingtin-interacting protein 2
    • HYPG
    • LIG
    • UBC1
    • UBE2K
    • UBE2K_HUMAN
    • Ubiquitin carrier protein
    • Ubiquitin conjugating enzyme E2 25 kDa
    • ubiquitin conjugating enzyme E2K
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast)
    • Ubiquitin protein ligase
    • Ubiquitin-conjugating enzyme E2 K
    • Ubiquitin-conjugating enzyme E2(25K)
    • Ubiquitin-conjugating enzyme E2-25 kDa
    • Ubiquitin-conjugating enzyme E2-25K
    • Ubiquitin-protein ligase
    see all
  • 機能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • 組織特異性Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • 翻訳後修飾Sumoylation at Lys-14 impairs catalytic activity.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant Human HIP2 protein (ab73665) 使用論文

ab73665 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab73665.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"