Recombinant Human HIF-2-alpha protein (ab114171)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号Q99814
    • 生物種Human
    • 配列SKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSEN ESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELT GHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVT NRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCE PIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSA YEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIY NPRNLQPQCIMCVNYVLSEI
    • 分子量61 kDa including tags
    • 領域28 to 347

特性

Our Abpromise guarantee covers the use of ab114171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

    ELISA

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • Basic helix loop helix PAS protein MOP2
    • Basic-helix-loop-helix-PAS protein MOP2
    • bHLHe73
    • Class E basic helix-loop-helix protein 73
    • ECYT4
    • Endothelial PAS domain containing protein 1
    • Endothelial pas domain protein 1
    • Endothelial PAS domain-containing protein 1
    • EPAS 1
    • EPAS-1
    • EPAS1
    • EPAS1_HUMAN
    • HIF 1 alpha like factor
    • HIF 2 alpha
    • HIF-1-alpha-like factor
    • HIF-2-alpha
    • HIF2-alpha
    • HIF2A
    • HLF
    • Hypoxia inducible factor 2 alpha
    • Hypoxia inducible factor 2 alpha subunit
    • Hypoxia-inducible factor 2-alpha
    • Member of PAS protein 2
    • Member of pas superfamily 2
    • MOP 2
    • MOP2
    • PAS domain-containing protein 2
    • PASD2
    see all
  • 機能Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
  • 組織特異性Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
  • 関連疾患Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻訳後修飾In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
    Phosphorylated on multiple sites in the CTAD.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant Human HIF-2-alpha protein 画像

  • ab114171 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

Recombinant Human HIF-2-alpha protein (ab114171) 使用論文

ab114171 has not yet been referenced specifically in any publications.

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