製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab42633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
  • アプリケーション

    Inhibition Assay

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • 備考Expressed in a Baculovirus infected Sf9 cell expression system. MW= 46.4 kDa. 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 138mM Sodium chloride, pH 8

関連情報

  • 別名
    • CDA07
    • CDLS5
    • HD 8
    • HD8
    • HDAC 8
    • Hdac8
    • HDAC8_HUMAN
    • HDACL 1
    • HDACL1
    • Histone deacetylase 8
    • Histone deacetylase like 1
    • MRXS6
    • RPD 3
    • RPD3
    • WTS
    see all
  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • 組織特異性Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • 配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻訳後修飾Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • 細胞内局在Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt

Recombinant Human HDAC8 protein (ab42633) 使用論文

ab42633 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab42633.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"