Recombinant human HDAC4 protein (ab104029)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号P56524
    • 生物種Human
    • 分子量77 kDa including tags
    • 領域612 to 1084

特性

Our Abpromise guarantee covers the use of ab104029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The Specific activity of ab104029 was determined to be 60 RLU/min/ng.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AHO3
    • BDMR
    • EC 3.5.1.98
    • HA6116
    • HD 4
    • HD4
    • HDAC 4
    • HDAC A
    • HDAC4
    • HDAC4_HUMAN
    • HDACA
    • Histone deacetylase 4
    • Histone Deacetylase A
    • KIAA0288
    see all
  • 機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR) [MIM:600430]. A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism.
  • 配列類似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • ドメインThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻訳後修飾Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
    Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
  • Information by UniProt

Recombinant human HDAC4 protein 画像

  • Kinase Assay demonstrating specific activity of ab104029.
  • SDS-PAGE showing ab104029 at approximately 77kDa.

Recombinant human HDAC4 protein (ab104029) 使用論文

ab104029 has not yet been referenced specifically in any publications.

Product Wall

Abcam's HDAC4 ab104029 is tested for activity using Promega's HDAC-Glo(TM) system. Here is a link: http://www.promega.com/resources/protocols/technical-manuals/101/hdac-glo-i-ii-assay-and-screening-system-protocol/ I hope this helps. If you have any ot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"