The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性Activity: 1100 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl,
pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI177), and 0.5 ng/ul HDAC2. Incubation condition: 20 min at 30 ºC.
備考Expressed in a Baculovirus infected Sf9 cell expression system.
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This product is an active protein and may elicit a biological response in vivo, handle with caution.
Histone deacetylase 2
Histone deacetylase 2 (HD2)
transcriptional regulator homolog RPD3
YY1 associated factor 1
YY1 transcription factor binding protein
機能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
組織特異性Widely expressed; lower levels in brain and lung.
配列類似性Belongs to the histone deacetylase family. HD type 1 subfamily.
翻訳後修飾S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.