Recombinant human Haspin protein (ab104026)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB
製品の詳細
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製品名
Recombinant human Haspin protein -
生理活性
The Specific activity of ab104026 was determined to be 18 nmol/min/mg.
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精製度
> 75 % SDS-PAGE.
Purity was determined to be >75% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
64 kDa including tags -
領域
470 to 798
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特性
Our Abpromise guarantee covers the use of ab104026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- germ cell associated 2
- Germ cell-specific gene 2 protein
- GSG2
see all -
機能
Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis. -
組織特異性
Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab104026 は論文での使用が確認できていません。