Recombinant human GSK3 beta protein (ab63193)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab63193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity: 168 nmol/min/mg.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態Liquid
  • 備考

    ab204860 (Glycogen synthase 1 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Glycogen Synthase Kinase 3 Beta
    • Glycogen synthase kinase-3 beta
    • GSK 3 beta
    • GSK-3 beta
    • GSK3B
    • GSK3B_HUMAN
    • GSK3beta isoform
    • Serine/threonine-protein kinase GSK3B
    see all
  • 機能Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • 組織特異性Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • 細胞内局在Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt

Recombinant human GSK3 beta protein 画像

  • Sample Kinase Activity Plot.
  • ab63193 on SDS-PAGE. Molecular Weight: ~48 kDa.

Recombinant human GSK3 beta protein (ab63193) 使用論文

ab63193 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab63193.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"