Recombinant human GDNF protein (ab73450)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

特性

Our Abpromise guarantee covers the use of ab73450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    Other

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    ab73450 is purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 150mM Sodium chloride, 10mM Sodium citrate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

関連情報

  • 別名
    • Astrocyte derived trophic factor
    • Astrocyte derived trophic factor 1
    • Astrocyte-derived trophic factor
    • Atf
    • ATF 1
    • ATF 2
    • ATF1
    • ATF2
    • gdnf
    • GDNF_HUMAN
    • Glial cell derived neurotrophic factor
    • Glial Cell Line Derived Neurotrophic Factor
    • Glial cell line-derived neurotrophic factor
    • Glial derived neurotrophic factor
    • HFB1 GDNF
    • hGDNF
    • HSCR3
    see all
  • 機能Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • 組織特異性In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • 関連疾患Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • 配列類似性Belongs to the TGF-beta family. GDNF subfamily.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant human GDNF protein 画像

  • Anti-GDNF antibody (ab28956) at 1/500 dilution + Recombinant human GDNF protein (ab73450) at 0.1 µg

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

Recombinant human GDNF protein (ab73450) 使用論文

ab73450 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Here is some further information about the two products:

ab73450 - http://www.uniprot.org/uniprot/P39905for the Swiss-Prot entry P39905
ab9790 - The ED50 was determined by the proliferation of rat...

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Thank you for contacting us.

The information we talked about over the phone can be found on the online datasheets. Please see the links below:

ab9790: http://www.abcam.com/gdnf-protein-ab9790.html

ab56286: http://www.abca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"