Recombinant human GDF8 / Myostatin protein (ab73251)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号O14793
    • 生物種Human
    • 配列The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.
    • 配列の追加情報The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp so starting at aa 267.

関連製品

特性

Our Abpromise guarantee covers the use of ab73251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    The ED50 as determined by the inhibition of the proliferation of MPC-11 cells is < 20ng/ml, corresponding to a Specific Activity of 50,000units/mg.

  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    This product is purified by proprietary chromatographic techniques. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 製品の状態Lyophilised
  • 備考For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None.
    Additives: None.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute in sterile 20mM HCl at 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

関連情報

  • 別名
    • GDF 8
    • GDF-8
    • GDF8
    • GDF8_HUMAN
    • Growth differentiation factor 8
    • Growth/differentiation factor 8
    • MSLHP
    • MSTN
    • Myostatin
    • OTTHUMP00000163498
    see all
  • 機能Acts specifically as a negative regulator of skeletal muscle growth.
  • 配列類似性Belongs to the TGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant human GDF8 / Myostatin protein (ab73251) 使用論文

ab73251 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We would suggest to reconstitute the sample in 20 mM HCL prior to diluting it. For our assay, the myostatin was not replenished everyday. Also, we are not sure of the stability in culture, but our assays are run f...

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Thank you for contacting us.

These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

I hope this information is helpful to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"