Recombinant human GDF8 / Myostatin protein (ab50097)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS
    • 分子量
      25 kDa
    • 領域
      267 to 375

関連製品

特性

Our Abpromise guarantee covers the use of ab50097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.

  • アプリケーション

    Inhibition Assay

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル
    < 0.100 Eu/µg
  • 精製度
    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.
  • 製品の状態
    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

関連情報

  • 別名
    • GDF 8
    • GDF-8
    • GDF8
    • GDF8_HUMAN
    • Growth differentiation factor 8
    • Growth/differentiation factor 8
    • MSLHP
    • MSTN
    • Myostatin
    • OTTHUMP00000163498
    see all
  • 機能
    Acts specifically as a negative regulator of skeletal muscle growth.
  • 配列類似性
    Belongs to the TGF-beta family.
  • 細胞内局在
    Secreted.
  • Information by UniProt

参考文献

ab50097 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.

These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

I hope this information is helpful to ...

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Thank you for your recent telephone enquiry. I am pleased to confirm I have obtained the original testing procedure for inhibition assays using ab50097 GDF8 / Myostatin protein. Cytotoxicity Assay for h-Myostatin using MPC-11 cells Procedur...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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