製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P55317
    • 生物種Human
    • 分子量49 kDa
    • 領域1 to 472

特性

Our Abpromise guarantee covers the use of ab98301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    SDS-PAGE

    Western blot

  • 精製度>= 80 % SDS-PAGE.
    ab98301 was expressed in E. coli and refolded to soluble form.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50mM HEPES, 5mM Calcium chloride, 3mM DTT, 500 mM L-arginine, 300 mM guanidine-HCl, 5 mM cystamine, 5 mM cysteamine, pH 7.5

関連情報

  • 別名
    • forkhead box A1
    • Forkhead box protein A1
    • FOX A1
    • FOXA1
    • FOXA1_HUMAN
    • hepatocyte nuclear factor 3 alpha
    • Hepatocyte nuclear factor 3-alpha
    • HNF 3A
    • HNF-3-alpha
    • HNF-3A
    • HNF3A
    • MGC33105
    • TCF 3A
    • TCF-3A
    • TCF3A
    • Transcription factor 3A
    see all
  • 機能Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • 組織特異性Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • 配列類似性Contains 1 fork-head DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant Human FOXA1 protein 画像

  • SDS-PAGE anaylsis of 5µg ab98301 stained with Coomassie dye.

Recombinant Human FOXA1 protein (ab98301) 使用論文

ab98301 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"