Recombinant human FKBP6 protein (ab95497)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, Functional Studies
製品の詳細
-
製品名
Recombinant human FKBP6 protein -
生理活性
Specific activity is > 190 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAPF-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Activity Assay
- Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
- Add 10 µl of recombinant FKBP6 protein with 1 µg in assay buffer.
- Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
- Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
- Record the increase in A405 nm for 30 minutes at 25°C.
-
精製度
> 95 % SDS-PAGE.
ab95497 is purified using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMGGSALNQGVLEGDDAPGQSLYERLSQRML DISGDRGVLKDVIREGAGDLVAPDASVLVKYSGYLEHMDRPFDSNYFRKT PRLMKLGEDITLWGMELGLLSMRRGELARFLFKPNYAYGTLGCPPLIPPN TTVLFEIELLDFLDCAESDKFCALSAEQQDQFPLQKVLKVAATEREFGNY LFRQNRFYDAKVRYKRALLLLRRRSAPPEEQHLVEAAKLPVLLNLSFTYL KLDRPTIALCYGEQALIIDQKNAKALFRCGQACLLLTEYQKARDFLVRAQ KEQPFNHDINNELKKLASCYRDYVDKEKEMWHRMFAPCGDGSTAGES -
予測される分子量
39 kDa including tags -
タグ
His tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab95497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Mass Spectrometry
Functional Studies
-
質量分析
MALDI-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.316% Tris HCl, 0.0292% EDTA, 40% Glycerol (glycerin, glycerine), 1.1688% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- 36 kDa FK506 binding protein
- 36 kDa FK506-binding protein
- 36 kDa FKBP
see all -
機能
PPIases accelerate the folding of proteins. -
組織特異性
Detected in all tissues examined, with higher expression in testis, heart, skeletal muscle, liver, and kidney. -
関連疾患
Note=FKBP6 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of FKBP6 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
配列類似性
Contains 1 PPIase FKBP-type domain.
Contains 3 TPR repeats. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab95497 は論文での使用が確認できていません。