製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MATGAEGKRKLQIGVKKRVDHCPIKSRKGDVLHMHYTGKLEDGTEFDSSL PQNQPFVFSLGTGQVIKGWDQGLLGMCEGEKRKLVIPSELGYGERGAPPK IPGGATLVFEVELLKIERRTEL

関連製品

特性

Our Abpromise guarantee covers the use of ab93681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity is > 270 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP2 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 90 % SDS-PAGE.
    ab93681 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 1mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 13 kDa FK506-binding protein
    • 13 kDa FKBP
    • EC 5.2.1.8
    • FK506 binding protein 2 (13kD)
    • FK506 binding protein 2 13kDa
    • FK506-binding protein 2
    • FK506-binding protein, T-cell, 13-KD
    • FKBP 13
    • FKBP 2
    • FKBP-13
    • FKBP-2
    • FKBP13
    • FKBP2
    • FKBP2_HUMAN
    • Immunophilin FKBP13
    • Peptidyl prolyl cis trans isomerase
    • Peptidyl-prolyl cis-trans isomerase FKBP2
    • PPIase
    • PPIase FKBP2
    • Proline isomerase
    • Rapamycin binding protein
    • Rotamase
    see all
  • 機能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 組織特異性T-cells and thymus.
  • 配列類似性Belongs to the FKBP-type PPIase family. FKBP2 subfamily.
    Contains 1 PPIase FKBP-type domain.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt

Recombinant human FKBP2 protein 画像

  • 15% SDS-PAGE analysis of ab93681 (3µg).

Recombinant human FKBP2 protein (ab93681) 使用論文

ab93681 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"