Recombinant human FKBP1B protein (ab113596)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P68106
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHRSMGVEIETISPGDGRTFPKKGQTCVVHYT GMLQNGKKFDSSRDRNKPFKFRIGKQEVIKGFEEGAAQMSLGQRAKLTCT PDVAYGATGHPGVIPPNATLIFDVELLNLE
    • 分子量14 kDa including tags
    • 領域1 to 108
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab113596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP1B protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • アプリケーション

    SDS-PAGE

    Functional Studies

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度> 90 % SDS-PAGE.
    ab113596 was purified using conventional chromatography.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.02% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 12.6 kDa FK506-binding protein
    • 12.6 kDa FKBP
    • Calstabin 2
    • FK506 binding protein 1 like
    • FK506 binding protein 12.6
    • FK506 binding protein 1B
    • FK506 binding protein 1B 12.6 kDa
    • FK506-binding protein 1B
    • FKB1B_HUMAN
    • FKBP 12.6
    • FKBP 1B
    • FKBP 1L
    • FKBP 9
    • FKBP-12.6
    • FKBP-1B
    • FKBP12.6
    • Fkbp1b
    • FKBP1L
    • FKBP9
    • h FKBP 12
    • h-FKBP-12
    • Immunophilin FKBP12.6
    • OTK 4
    • OTK4
    • Peptidyl prolyl cis trans isomerase
    • Peptidyl prolyl cis trans isomerase 1B
    • Peptidyl-prolyl cis-trans isomerase FKBP1B
    • PKBP 1L
    • PKBP1L
    • PPIase
    • PPIase 1B
    • PPIase FKBP1B
    • Rotamase
    • Rotamase 1B
    see all
  • 機能Associates with the ryanodine receptor (RYR-2) in cardiac muscle sarcoplasmic reticulum and may play a unique physiological role in excitation-contraction coupling in cardiac muscle. There are four molecules of FKBP12.6 per heart muscle RYR. Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 組織特異性Isoform 1 and isoform 2 are Ubiquitous with highest levels in brain and thymus.
  • 配列類似性Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
    Contains 1 PPIase FKBP-type domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant human FKBP1B protein 画像

  • 15% SDS-PAGE analysis of ab113596 (3µg)

Recombinant human FKBP1B protein (ab113596) 使用論文

ab113596 has not yet been referenced specifically in any publications.

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