Recombinant human Fibroblast activation protein, alpha (ab79623)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • 領域29 to 760
    • タグHis tag C-Terminus

特性

Our Abpromise guarantee covers the use of ab79623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific Activity: 10.2 pmol/min/µg. Assay Condition: 50 mM Tris-HCl (pH7.5), 10 mM MgCl2, 1 mM MnCl2, 5µM substrate (A-P-AMC), room temperature.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.36% Imidazole
    Constituents: 0.64% Tris HCl, 20% Glycerol, 0.64% Sodium chloride, 0.02% Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 170 kDa melanoma membrane bound gelatinase
    • 170 kDa melanoma membrane-bound gelatinase
    • DPPIV
    • FAP
    • FAPA
    • Fibroblast activation protein alpha
    • Integral membrane serine protease
    • SEPR_HUMAN
    • Seprase
    see all
  • 機能In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • 組織特異性Fibroblast specific.
  • 配列類似性Belongs to the peptidase S9B family.
  • 翻訳後修飾N-glycosylated.
    The N-terminus may be blocked.
  • 細胞内局在Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt

Recombinant human Fibroblast activation protein, alpha 画像

  • Image showing specific activity of ab79623.
  • SDS-PAGE showing 3ug ab79623 at approximately 85.5kDa.

Recombinant human Fibroblast activation protein, alpha (ab79623) 使用論文

ab79623 has not yet been referenced specifically in any publications.

Product Wall



The Active human Fibroblast activation protein, alpha protein fragment (ab79623) contains a His tag at the C terminal end.

Thank you for your enquiry and interest in our products.

I would suggest loading between 1-5 ug per lane but this may need to be optimized since it depends on the detection system you will apply. Please take a look at the SDS-PAGE image on th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"