Recombinant Human FGFR1 alpha protein (Fc Chimera) (ab84000)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P11362
    • 生物種Human
    • 配列Theoretical Sequence: RPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDD VQSINWLRDGVQLAESNRTRITGE EVEVQDSVPADSGLYACVTSSPSG SDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKP NRMPVAPYWT SPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGY KV RYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHR PILQAGLPANKTVALGSN VEFMCKVYSDPQPHIQWLKHIEVNGSKIGP DNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDA GEYTCLAGNSIGLS HHSAWLTVLEALEGIPKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKP REEQYNSTYRVVSVL TVLHQDWLNGKEYKCRVSNKALPAPIEKTISKA KGQPREPQVYTLPPSRDELTKNQVSLTCLVK GFYPSDIAVEWESNGQP ENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL HNH YTQKSLSLSPGK
    • 領域1 to 363
    • 配列の追加情報Encodes the signal peptide and extracellular domain of human FGF R1 alpha (IIIc) (aa 1-363) was fused to the Fc region IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.

関連製品

特性

Our Abpromise guarantee covers the use of ab84000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • BFGFR
    • CD331
    • CEK
    • FGFBR
    • FGFR1
    • FLG
    • FLT2
    • FMS like tyrosine kinase 2
    • HBGFR
    • KAL2
    • N SAM
    • OGD
    see all
  • 関連性FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
  • 細胞内局在Cell Membrane

Recombinant Human FGFR1 alpha protein (Fc Chimera) 画像

  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab84000 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab84000
    Lane 2: ab84000 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab84000 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    10 µg protein was loaded per lane; Gel was stained with Deep Purple™.
  • A sample of ab84000 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein was loaded. Spot train (Deep Purple™ stained) indicates presence of multiple isoforms.

Recombinant Human FGFR1 alpha protein (Fc Chimera) (ab84000) 使用論文

ab84000 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. When reconstituted as directed, the solution is in PBS with 1% human serum albumin and 10% trehalose, which are added as stabilizers, thus the specific concentration of the target can no longer be measured by OD 280 or any...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"