Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)

製品の概要

  • 製品名
    Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active)
  • タンパク質長
    Full length protein
  • 製品の詳細
    Recombinant human FGF21 + IgG1 protein (Fc Chimera Active)

製品の詳細

  • 由来
    Recombinant
  • 由来
    HEK 293 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      50 kDa including tags
    • 配列の追加情報
      Human FGF21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1.

特性

Our Abpromise guarantee covers the use of ab108556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • エンドトキシン・レベル
    < 0.100 Eu/µg
  • 精製度
    >= 95 % SDS-PAGE.
    ab108556 is 0.2 µm filtered.
  • 製品の状態
    Liquid
  • 備考
    Working aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 細胞内局在
    FGF21: Secreted. IgG1: Secreted. Cell membrane; Single-pass membrane protein

画像

  • ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
    bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
    Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.

    Lane 1: 1 µg/ml hFGF21-His (competitor)
    Lane 2: 2.2 µg/ml ab108556 (hFGF21-Fc)
    Lane 3: 2.2 µg/ml mCD137-Fc
    Lane 4: 100 ng/µl FGF-b
    Lane 5: control (non-treated)

参考文献

ab108556 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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