The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性Biological Activity: Measured by its ability to stimulate proliferation of NIH3T3 cells.
The ED50 of this effect is between 0.1 - 1 ng/ml.
% SDS-PAGE. Purity: >98% by SDS-PAGE and HPLC analysis.1EU per ug of bFGF as determined by the LAL method
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成To resconstitute the protein add sterile water
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
Fibroblast growth factor 2
Fibroblast growth factor 2 (basic)
Fibroblast growth factor, basic
Heparin binding growth factor 2 precursor
Heparin-binding growth factor 2
機能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
配列類似性Belongs to the heparin-binding growth factors family.
翻訳後修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
細胞内局在Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.