製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

特性

Our Abpromise guarantee covers the use of ab61845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity of 3MIU/mg
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    Purity greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
  • 製品の状態Lyophilised
  • 備考Add 0.1% HSA or BSA for long term storage at -20°C.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Sodium phosphate, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

関連情報

  • 別名
    • Basic fibroblast growth factor
    • Basic fibroblast growth factor bFGF
    • BFGF
    • FGF 2
    • FGF B
    • FGF-2
    • Fgf2
    • FGF2 basic
    • FGF2_HUMAN
    • FGFB
    • Fibroblast growth factor 2
    • Fibroblast growth factor 2 (basic)
    • Fibroblast growth factor, basic
    • HBGF 2
    • HBGF-2
    • HBGF2
    • HBGH 2
    • HBGH2
    • Heparin binding growth factor 2 precursor
    • Heparin-binding growth factor 2
    • Prostatropin
    see all
  • 機能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 組織特異性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 配列類似性Belongs to the heparin-binding growth factors family.
  • 翻訳後修飾Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 細胞内局在Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

Recombinant human FGF2 protein 画像

  • Dose-dependent proliferation of mouse BALB/c 3T3 cells with ab61845. Assays were carried out in triplicate.

  • ab61845 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human FGF basic has a predicted MW of 16.5 kDa.

Recombinant human FGF2 protein (ab61845) 使用論文

ab61845 has not yet been referenced specifically in any publications.

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