Recombinant human FGF16 protein (ab9865)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human FGF16 protein -
生理活性
Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).
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精製度
> 95 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per g (1EU/g). -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MAEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR -
予測される分子量
24 kDa
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特性
Our Abpromise guarantee covers the use of ab9865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
Human FGF-16 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg For most in vitro applications, FGF-16 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成For initital reconstitution, please reconstitue this product in water to 0.1 - 1 mg/mL. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
関連情報
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別名
- FGF 16
- FGF-16
- Fgf16
see all -
機能
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab9865 は論文での使用が確認できていません。