Recombinant Human FEN1 protein (ab95382)

製品の概要

  • 製品名Recombinant Human FEN1 protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK

特性

Our Abpromise guarantee covers the use of ab95382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • 精製度> 90 % SDS-PAGE.
    ab95382 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

関連情報

  • 別名
    • DNase IV
    • FEN-1
    • FEN1
    • FEN1_HUMAN
    • Flap endonuclease 1
    • Flap structure specific endonuclease 1
    • Flap structure-specific endonuclease 1
    • hFEN-1
    • hFEN1
    • Maturation factor 1
    • MF1
    • Rad2
    see all
  • 機能Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • 配列類似性Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
  • 翻訳後修飾Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
    Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
    Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
  • 細胞内局在Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
  • Information by UniProt

Recombinant Human FEN1 protein 画像

  • 15% SDS-PAGE analysis of 3µg ab95382.
  • Anti-FEN1 antibody (ab70815) at 1 µg/ml + Recombinant Human FEN1 protein (ab95382) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 150 seconds

Recombinant Human FEN1 protein (ab95382) 使用論文

ab95382 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The lab has confirmed that this protein is not tagged. The sequence of this protein is : MGIQGLAKLI ADVAPSAIR E NDIKSYFG K VAIDASMS Y QFLIAVRQ G DVLQNEEG T TSHLMGMF R TIRMMENG K PVYVFDGK P QLKSGELA R SERRAEAE Q LQQ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"