Recombinant Human Estrogen Receptor alpha protein (ab82606)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human Estrogen Receptor alpha protein
Estrogen Receptor alpha タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab82606 is greater than 90% homogeneous and based on SDS-PAGE analysis, purified by affinity and FPLC chromatography. -
発現系
Baculovirus infected insect cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA VYNYPEGAAY EFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL NSVSPSPLML LHPPPQLSPF LQPHGQQVPY YLENEPSGYT VREAGPPAFY RPNSDNRRQG GRERLASTND KGSMAMESAK ETRYCAVCND YASGYHYGVW SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQAC RLRKCYEVGM MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKR SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV -
予測される分子量
68 kDa -
領域
1 to 595 -
タグ
His tag N-Terminus
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関連製品
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Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)
- Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063)
- Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Estrogen Receptor alpha antibody (ab75635)
特性
Our Abpromise guarantee covers the use of ab82606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.00584% EDTA, 0.316% Tris HCl, 0.0154% DTT, 0.75% Potassium chloride
関連情報
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別名
- 7*/654 isoform
- 7*/819 2 isoform
- 7*/822 isoform
see all -
機能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab82606 は 3 報の論文で使用されています。
- Vanzan L et al. High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites. Nat Commun 12:3337 (2021). PubMed: 34099689
- Tian Z et al. TRIM8 inhibits breast cancer proliferation by regulating estrogen signaling. Am J Cancer Res 10:3440-3457 (2020). PubMed: 33163282
- Xue M et al. Regulation of estrogen signaling and breast cancer proliferation by an ubiquitin ligase TRIM56. Oncogenesis 8:30 (2019). PubMed: 31000690