Recombinant Human ERK2 protein (ab43625)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab43625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    Western blot

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

関連情報

  • 別名
    • ERK
    • ERK 2
    • ERK-2
    • ERT1
    • Extracellular Signal Regulated Kinase 2
    • Extracellular signal-regulated kinase 2
    • MAP kinase 1
    • MAP kinase 2
    • MAP kinase isoform p42
    • MAPK 1
    • MAPK 2
    • Mapk1
    • MAPK2
    • Mitogen-activated protein kinase 1
    • Mitogen-activated protein kinase 2
    • MK01_HUMAN
    • P38
    • P40
    • P41
    • p42-MAPK
    • P42MAPK
    • PRKM1
    • PRKM2
    • protein kinase, mitogen-activated, 1
    • protein kinase, mitogen-activated, 2
    • protein tyrosine kinase ERK2
    see all
  • 機能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant Human ERK2 protein 画像

  • The purity of ab43625 was determined to be >90% by SDS-PAGE. MW ~42kDa.

Recombinant Human ERK2 protein (ab43625) 使用論文

ab43625 has not yet been referenced specifically in any publications.

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Vielen Dank für Ihren Anruf.

Das Protein im Röhrchen selber wurde nach der Produktion in E. coli nicht aktiviert. Das Labor hat jedoch erfolgreich die Aktivität dieses Proteins demonstriert nach Aktivierung desselben mit MEK kinase.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"