Recombinant Human Endothelin B Receptor protein (ab114259)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号P24530
    • 生物種Human
    • 配列EERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAEVPKGDR TAGSPPRTISPPPCQGPIEIKETFK
    • 分子量34 kDa including tags
    • 領域27 to 101

関連製品

特性

Our Abpromise guarantee covers the use of ab114259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 µg/µl
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • ABCDS
    • Ednra
    • EDNRB
    • EDNRB_HUMAN
    • Endothelin B receptor
    • Endothelin B receptor precursor
    • Endothelin receptor Non selective type
    • Endothelin receptor non-selective type
    • Endothelin receptor type B
    • ET B
    • ET-B
    • ET-BR
    • ETB
    • ETBR
    • ETRB
    • Hirschsprung disease 2
    • HSCR
    • HSCR2
    • OTTHUMP00000018534
    • OTTHUMP00000178736
    • WS4A
    see all
  • 機能Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 組織特異性Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • 関連疾患Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • 翻訳後修飾Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • 細胞内局在Cell membrane.
  • Information by UniProt

Recombinant Human Endothelin B Receptor protein 画像

  • ab114259 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Recombinant Human Endothelin B Receptor protein (ab114259) 使用論文

ab114259 has not yet been referenced specifically in any publications.

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Thank you for your reply.


Both recombinant fragment peptide are validated for ELISA, but the protocol used will depend largely on the antibodies being used in the assay. The data I sent previously included relevant protocol information....

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Thank you for your reply.


Both proteins have been used in different ELISA assays:


Regarding ab114239, this was tested in a sELISA. On the other hand, ab114259 was tested in a simple ELISA assay in which the protein was c...

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Thank you for contacting Abcam regarding the proteins ab114239 and ab114259.


I have reviewed the product specifications of these proteins as well as the kit protocols the customer is using. It is important to note that both of these pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"