Recombinant human EGFR protein (ab89746)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Insect cells
  • アミノ酸配列
    • 生物種Human
    • 領域695 to 1210

特性

Our Abpromise guarantee covers the use of ab89746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The Specific activity of ab89746 was determined to be 60 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

  • 製品の状態Liquid
  • 備考

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog
    • Cell growth inhibiting protein 40
    • Cell proliferation inducing protein 61
    • EGF R
    • EGFR
    • EGFR_HUMAN
    • Epidermal growth factor receptor
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)
    • erb-b2 receptor tyrosine kinase 1
    • ERBB
    • ERBB1
    • Errp
    • HER1
    • mENA
    • NISBD2
    • Oncogen ERBB
    • PIG61
    • Proto-oncogene c-ErbB-1
    • Receptor tyrosine protein kinase ErbB 1
    • Receptor tyrosine-protein kinase ErbB-1
    • SA7
    • Species antigen 7
    • Urogastrone
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog
    • wa2
    • Wa5
    see all
  • 機能Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • 組織特異性Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • 関連疾患Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
  • 細胞内局在Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt

Recombinant human EGFR protein 画像

  • SDS-PAGE showing ab89746 at approximately 89kDa.
  • Kinase Assay demonstrating specific activity of ab89746.

Recombinant human EGFR protein (ab89746) 使用論文

ab89746 has not yet been referenced specifically in any publications.

Product Wall

If your sample is 90% pure EGFR, then a concentration of 2 ug/ml will give you a signal. You may find that less is sufficient, so I recommend testing a range, such as 0.125, 0.5, and 2 ug/ml. The note in the protocol recommending 20 ug/ml refers to tot...

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That is correct, amino acid range aa 695-1210 is a cytoplasmic (i.e., intracellular) domain. The numbering refers to the sequence at the following UniProt entry, and a list of the domains and the amino acid ranges that constitute these domains is about...

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No, the expression host, virus-infected insect cells, will have no bearing on the reactivity in this case. I recommend choosing an antibody that is known to react with EGFR in its native conformation, as demonstrated, for instance, by immunocytochemist...

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It is very likely that ab89746 can be used in a direct ELISA, assuming you try to detect it with an antibody that is raised against an amino acid sequence that is present in ab89746, which is a recombinant fragment of full-length EGFR, from amino acid ...

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I am very pleased to hear you would like to accept our offer and test ab89746 in sELISA. This code will give you 1 freePROTEIN before the expiration ...

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I have received more information from my colleague which I would like to share with you. Unfortunately they have recommended not using ab89746 because ligand receptor association cannot be achieved extracellularly due to the hydrophilic nature of t...

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Thank you for contacting us. We have not tested these products in Biocore (surface plasmon resonance) experiments. Theoretically these products should work however the conditions have to be optimized by end user. We can recommend coating ligand...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this protein. In answer to your question, I am contacting our source to obtain the testing procedure for you. We will hap...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"