製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Pichia pastoris
  • アミノ酸配列
    • 生物種
      Human
    • 配列
      The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF. N-terminal methionine has been completely removed enzymatically.

特性

Our Abpromise guarantee covers the use of ab55483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

    Functional Studies

  • 精製度
    > 95 % SDS-PAGE.
    ab55483 was purified by chromatographic techniques. Purity tested by SDS PAGE and RP-HPLC.
  • 製品の状態
    Lyophilised
  • 備考
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 150mM Sodium chloride, 25mM Sodium bicarbonate, pH 7.5

  • 再構成
    It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

関連情報

  • 別名
    • Beta urogastrone
    • beta-urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • 機能
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 組織特異性
    Expressed in kidney, salivary gland, cerebrum and prostate.
  • 関連疾患
    Hypomagnesemia 4
  • 配列類似性
    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻訳後修飾
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 細胞内局在
    Membrane.
  • Information by UniProt

参考文献

ab55483 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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