Recombinant Human E2F1 protein (ab82207)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, GSA, Functional Studies
製品の詳細
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製品名
Recombinant Human E2F1 protein -
精製度
> 95 % SDS-PAGE.
Purified by a combination of affinity chromatography and FPLC chromatography. -
発現系
Baculovirus infected insect cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab82207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Gel Supershift Assays
Functional Studies
EMSA
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
関連情報
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別名
- Dmel\CG6376
- Dmel_CG6376
- drosE2F1
see all -
機能
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
配列類似性
Belongs to the E2F/DP family. -
翻訳後修飾
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab82207 は 1 報の論文で使用されています。
- Vanzan L et al. High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites. Nat Commun 12:3337 (2021). PubMed: 34099689