The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Reconstitute with 50 µl sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial. After reconsitution short term store at 4 °C, for long term storage store aliquoted at - 20 °C
Cytotoxic TRAIL receptor
Death receptor 4
NF related apoptosis-inducing ligand receptor 1
TNF receptor superfamily member 10a
TNF-related apoptosis-inducing ligand receptor 1
TRAIL receptor 1
Tumor necrosis factor receptor superfamily member 10A
Tumor necrosis factor receptor superfamily member 10a variant 2
Tumor necrosis factor receptor superfamily, member 10a
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
Contains 1 death domain. Contains 3 TNFR-Cys repeats.
SDS Page analysis of ab56518; 12% separating gel; reducing conditions.
Lane 1: 2 µg,
Lane 2: 1 µg,
Lane 3: 0.5 µg.
Staining with Coomasie Blue.
Western blot - DR4 + Fc Fragment protein (ab56518)
ab56518 has a calculated molecular mass of 43 kDa. As a result of glycosylation, the recombinant protein migrates approximately as a 46 kDa protein in SDS-PAGE under reducing conditions.
Immunostaining with DR4 specific antibody, detection with Goat anti-mouse / HRP secondary antibody. Gel: 12%.
Lane 1 : ab56518 at 0.37 µg,
Lane 2 : ab56518 at 0.15 µg,
Lane 3 : ab56518 at 0.075 µg,
Lane 4 : ab56518 at 0.037 µg,
Lane 5 : ab56518 at 0.015 µg.
Recombinant Human DR4 protein (ab56518) 使用論文
has not yet been referenced specifically in any publications.