Recombinant Human DNA polymerase alpha protein (ab114839)

製品の概要

  • 製品名Recombinant Human DNA polymerase alpha protein
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号P09884
    • 生物種Human
    • 配列QFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDH EKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS
    • 分子量37 kDa including tags
    • 領域1363 to 1462

関連製品

特性

Our Abpromise guarantee covers the use of ab114839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • DKFZp686K1672
    • DNA polymerase alpha 1 catalytic subunit
    • DNA polymerase alpha catalytic subunit
    • DNA polymerase alpha catalytic subunit p180
    • DNA polymerase alpha p180 subunit
    • DPOLA_HUMAN
    • OTTHUMP00000023089
    • OTTHUMP00000023090
    • P180
    • p180 subunit
    • POLA
    • POLA1
    • Polymerase (DNA directed), alpha 1, catalytic subunit
    • Polymerase (DNA-directed), alpha (70kD)
    see all
  • 機能Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
  • 配列類似性Belongs to the DNA polymerase type-B family.
  • 翻訳後修飾A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant Human DNA polymerase alpha protein 画像

  • ab114839 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Recombinant Human DNA polymerase alpha protein (ab114839) 使用論文

ab114839 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"