Recombinant Human Crk p38 protein (ab101046)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human Crk p38 protein -
精製度
> 95 % SDS-PAGE.
ab101046 was purified by using conventional chromatography. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMAGNFDSEERSSWYWGRLSRQEAVALLQGQ RHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPP GVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILR QEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRG MIPVPYVEKYRPASASVSALIGGR -
予測される分子量
25 kDa -
領域
1 to 224 -
タグ
His tag N-Terminus
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関連製品
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Positive Controls
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Related Products
特性
Our Abpromise guarantee covers the use of ab101046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Mass Spectrometry
SDS-PAGE
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質量分析
MALDI-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
関連情報
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別名
- Adapter molecule crk
- Avian sarcoma virus CT10 (v crk) oncogene homolog
- CRK
see all -
機能
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. -
配列類似性
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
ドメイン
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB. -
翻訳後修飾
Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. -
細胞内局在
Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab101046 は論文での使用が確認できていません。