Recombinant human Cripto1 protein (Fc Chimera) (ab84062)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P13385
    • 生物種Human
    • 配列Theoretical sequence: LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPP MGIQHSKEL NRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGS VPHDTWLPK KCSLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPE LPPSGSSNT KVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTP EVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQY NSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR EPQVYTLPP SRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYK TTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK
    • 領域31 to 169
    • 配列の追加情報Encodes the signal peptide and extracellular domain of human Cripto-1 (aa 1-169) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modified human 293 cells.

特性

Our Abpromise guarantee covers the use of ab84062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性200 ng/ml of this Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells.
  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考 200 ng/ml of this Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • CR
    • CRGF
    • cripto
    • Cripto 1
    • Cripto 1 growth factor
    • Cripto-1 growth factor
    • Cripto1 growth factor
    • Epidermal growth factor like cripto protein CR1
    • Epidermal growth factor-like cripto protein CR1
    • TDGF 1
    • TDGF1
    • TDGF1_HUMAN
    • Teratocarcinoma derived growth factor 1
    • Teratocarcinoma-derived growth factor 1
    see all
  • 機能Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • 組織特異性Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • 配列類似性Contains 1 EGF-like domain.
  • 細胞内局在Cell membrane.
  • Information by UniProt

Recombinant human Cripto1 protein (Fc Chimera) 画像

  • Lane 1 – MW markers; Lane 2 – ab84062; Lane 3 – ab84062 treated with PNGase F to remove potential N linked glycans; Lane 4 – ab84062 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
    Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
  • A sample of ab84062 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4 20% Tris-HCl 2D gel. Approximately 40 μg of protein was loaded; Gel was stained using Deep Purple™. The spot train indicates the presence of multiple glycoforms. Spots within the spot train were cut from the gel and identified as Cripto1 (Fc Chimera) by protein mass fingerprinting.
  • Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
    The triangle indicates theoretical pI and MW of the protein.

Recombinant human Cripto1 protein (Fc Chimera) (ab84062) 使用論文

ab84062 has not yet been referenced specifically in any publications.

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