Recombinant Human Cofilin protein (ab85154)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human Cofilin protein
Cofilin-1 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % Densitometry. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Recombinant Protein
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Related Products
特性
Our Abpromise guarantee covers the use of ab85154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.0308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
関連情報
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別名
- 18 kDa phosphoprotein
- CFL
- CFL 1
see all -
機能
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
組織特異性
Widely distributed in various tissues. -
配列類似性
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
翻訳後修飾
Phosphorylated on Ser-3 in resting cells. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85154 は 1 報の論文で使用されています。
- Lu H et al. Curcolonol suppresses the motility of breast cancer cells by inhibiting LIM kinase 1 to downregulate cofilin 1 phosphorylation. Int J Oncol 53:2695-2704 (2018). PubMed: 30320377