Recombinant human CLK3 protein (ab85759)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human CLK3 protein -
生理活性
The Specific activity of ab85759 was determined to be 75 nmol/min/mg.
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精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab85759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- CDC like kinase 3
- CDC-like kinase 3
- CLK3
see all -
機能
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylates on all three types of residues. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. - Information by UniProt
画像
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The specific activity of CLK3 (ab85759) was determined to be 28.9 nmol/min/mg as per activity assay protocol and was equivalent to 80 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab85759
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The Specific activity of ab85759 was determined to be 75 nmol/min/mg.
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SDS-PAGE showing ab85759 at approximately 86kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab85759 は論文での使用が確認できていません。