Recombinant Human CLIC4 protein (ab104744)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号Q9Y696
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHMALSMPLNGLKEEDKEPLIELFVKAGSDGE SIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITF NSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIK NSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLD GNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGIWRYLTNAYSRDEF TNTCPSDKEVEIAYSDVAKRLTK
    • 分子量31 kDa including tags
    • 領域1 to 253
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab104744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Mass Spectrometry

    SDS-PAGE

  • 質量分析
    MALDI-TOF
  • 精製度> 95 % SDS-PAGE.
    ab104744 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

関連情報

  • 別名
    • Chloride intracellular channel 4
    • Chloride intracellular channel 4 (mitochondrial)
    • Chloride intracellular channel 4 like
    • Chloride intracellular channel protein 4
    • Clic4
    • CLIC4_HUMAN
    • CLIC4L
    • DKFZP566G223
    • FLJ38640
    • H1
    • HUH1
    • Intracellular chloride ion channel protein p64H1
    • MC3S5
    • mtCLIC
    • p64H1
    see all
  • 機能Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
  • 組織特異性Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.
  • 配列類似性Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • ドメインMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • 細胞内局在Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane. Nucleus matrix. Cell membrane. Mitochondrion. Cell junction. Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.
  • Information by UniProt

Recombinant Human CLIC4 protein 画像

  • 15% SDS-PAGE (3µg).

Recombinant Human CLIC4 protein (ab104744) 使用論文

ab104744 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Used this purified protein as a positive control to determine antibody specificity to CLIC4 in western blots. Very low amount (0.5 ug/lane) of protein was sufficient to yield a positive result. Purified CLIC1 protein (ab95486) from Abcam was used as a negative control.
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投稿 Jun 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"