Recombinant Human Claspin protein (ab125977)

製品の概要

  • 製品名Recombinant Human Claspin protein
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号Q9HAW4
    • 生物種Human
    • 分子量24 kDa including tags
    • 領域1126 to 1330

特性

Our Abpromise guarantee covers the use of ab125977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • 再構成Reconstitute with water to desired concentration

関連情報

  • 別名
    • Claspin
    • Claspin homolog
    • claspin homolog (Xenopus laevis)
    • CLSPN
    • CLSPN protein
    • CLSPN_HUMAN
    • hClaspin
    • Hu Claspin
    see all
  • 機能Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • 配列類似性Belongs to the claspin family.
  • ドメインThe C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • 翻訳後修飾Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant Human Claspin protein (ab125977) 使用論文

ab125977 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"