The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab124314 has full biological activity when compared to standards. The ED50, as determined by its ability to chemoattract Human Chem23R transfected BaF3 mouse pro-B cells, is typically 4-20 ng/mL.
% SDS-PAGE. Purity by SDS-PAGE: =98%
Purity by HPLC: =98%
Endotoxin level: <0.01 ng/µg
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Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituent: 0.2% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge vial before opening. When reconstituting the product, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions.
Zhang Y et al. Chemerin suppresses neuroinflammation and improves neurological recovery via CaMKK2/AMPK/Nrf2 pathway after germinal matrix hemorrhage in neonatal rats. Brain Behav Immun70:179-193 (2018).
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