Recombinant human CDK6 + CCND1 protein (ab84559)

製品の概要

  • 製品名Recombinant human CDK6 + CCND1 protein
  • タンパク質長Full length protein

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab84559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 製品の状態Liquid
  • 備考

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • B cell CLL/lymphoma 1
    • BCL 1 oncogene
    • BCL1
    • cell division protein kinase 6
    • cyclin D1 1
    • cyclin dependent kinase 6
    • G1 S specific cyclin D1
    • PRAD1
    • PRAD1 oncogene
    • Serine threonine protein kinase PLSTIRE
    see all
  • 関連性CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2). CCND1 is essential for the control of the cell cycle at the G1/S (start) transition

Recombinant human CDK6 + CCND1 protein 画像

  • Purity of CDK6 + CCND1 was determined to be >85% by densitometry. CDK6 approx. MW 40kDa and CCND1 approx. MW 61kDa.
  • Specific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.

Recombinant human CDK6 + CCND1 protein (ab84559) 使用論文

ab84559 has not yet been referenced specifically in any publications.

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