Recombinant Human CDK5R1 protein (ab125653)

製品の概要

  • 製品名
    Recombinant Human CDK5R1 protein
  • タンパク質長
    Full length protein

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      49 kDa including tags
    • 領域
      98 to 307

特性

Our Abpromise guarantee covers the use of ab125653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 精製度
    > 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

関連情報

  • 別名
    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5R1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • p23
    • p25
    • p35
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • 機能
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 組織特異性
    Brain and neuron specific.
  • 配列類似性
    Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻訳後修飾
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 細胞内局在
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

画像

  • SDS-PAGE analysis of ab125653.

参考文献

ab125653 has not yet been referenced specifically in any publications.

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