Recombinant Human Cdk5 protein (ab56282)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号NM_004935
    • 生物種Human
    • 分子量58 kDa
    • タグproprietary tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab56282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    Western blot

    SDS-PAGE

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

関連情報

  • 別名
    • Cdk 5
    • Cdk5
    • CDK5_HUMAN
    • Cell division protein kinase 5
    • Crk6
    • Cyclin dependent kinase 5
    • Cyclin-dependent kinase 5
    • Protein kinase CDK5 splicing
    • PSSALRE
    • Serine threonine protein kinase PSSALRE
    • Serine/threonine-protein kinase PSSALRE
    • Tau protein kinase II catalytic subunit
    • TPKII catalytic subunit
    see all
  • 機能Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
  • Information by UniProt

Recombinant Human Cdk5 protein 画像

Recombinant Human Cdk5 protein (ab56282) 使用論文

ab56282 has not yet been referenced specifically in any publications.

Product Wall

I just am writring to let you know that the active protein is on our catalog. The product ID is ab123653 and named p35 + p25 protein (Tagged).

Application Western blot

Review text: The blot image provided in this review has mouse Dorsal Striatum loaded at 70ug in lanes 2-7 and the CDK5-GST lysate in 1, 3 and 5 ug in lanes 8-10. The amount of CDK5-GST should be used at a 10th of that shown here, namely .1, .3 and .5 ug as the amount used was unnecessarily high. A goat polyclonal anti-GST antibody was used from GE Health sciences. Therefore, lanes 8-10 have a yellow band because of co-labeling of CDK5 and the GST tag. There was some nonspecific binding with the CDK5-GST lysate due to such a high amount being used. There was also nonspecific binding with the anti-GST antibody within the CDK5-GST lysate but not the Dorsal Striatum lysates.
Sample: Mouse Tissue lysate - whole (Dorsal Striatum)
Loading amount: 70 µg
Specification: Dorsal Striatum
Gel Running Conditions: Reduced Denaturing (10% gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details
Incubation time: 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk

Primary antibody (in addition to 'Cdk5 protein (Tagged)')
Name: Non-Abcam antibody was used: CDK5 (sc-173)
Host species: Rabbit
Clonality: Polyclonal
Dilution: 1/2000

Secondary antibody
Name: Non-Abcam antibody was used: Licor 700
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX

Detection
Detection method: Licor
Exposure: 10 minute(s) and 0 second(s)
Bands: Specific: 32 and 56 kDa Non-specific: 37 kDa
Positive control: The CDK5-GST lysate was the positive control.
Negative control: None.
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投稿 Mar 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"