Recombinant human CDK2 + CCNE1 protein (ab85639) (ab85639)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human CDK2 + CCNE1 protein (ab85639) -
生理活性
The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
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精製度
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
タグ
GST tag N-Terminus
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アミノ酸配列 2
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生物種
Human -
タグ
GST tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab85639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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細胞内局在
Cyclin E1: Nucleus.
画像
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The specific activity of CDK2 + CCNE1 (ab85639) was determined to be 345 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab85639
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SDS PAGE analysis of ab85639
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The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
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SDS-PAGE showing ab85639 at approximately 58kDa (CDK2) and 73kDa (CCNE1).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab85639 は 3 報の論文で使用されています。
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). PubMed: 29853338
- Azzarelli R et al. Multi-site Neurogenin3 Phosphorylation Controls Pancreatic Endocrine Differentiation. Dev Cell 41:274-286.e5 (2017). PubMed: 28457793
- Kitagawa K et al. Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development. Mol Cell Biol 34:2732-44 (2014). PubMed: 24820417