Recombinant Human CDC42 protein (ab87713)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human CDC42 protein
CDC42 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
ab87713 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method). -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab87713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0584% EDTA, 10% Glycerol (glycerin, glycerine)
関連情報
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別名
- CDC42
- CDC42_HUMAN
- CDC42Hs
see all -
機能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
翻訳後修飾
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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15% SDS-PAGE showing ab87713 at approximately 22.4kDa (3µg).
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All lanes : Anti-CDC42 antibody (ab64533) at 1 µg/ml
Lane 1 :Recombinant Human CDC42 protein (ab87713) at 0.1 µg
Lane 2 :Recombinant Human CDC42 protein (ab87713) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab87713 は論文での使用が確認できていません。