製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human
    • 領域584 to 1256

特性

Our Abpromise guarantee covers the use of ab42584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific Activity: 26 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM pNPP. The specific activity of CD45 was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 2 min incubation at 30°C, 2µg/ml enzyme.
  • アプリケーション

    Functional Studies

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • 備考Protein was expressed in a Baculovirus Sf9 expression system. Expected molecular weight 75kDa.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 75mM Sodium chloride, 5mM DTT, 25mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • B220
    • CD 45
    • CD45
    • CD45 antigen
    • CD45R
    • GP180
    • L-CA
    • LCA
    • Leukocyte common antigen
    • loc
    • Ly-5
    • LY5
    • Ly5, homolog of
    • Lyt-4
    • OTTHUMP00000033813
    • OTTHUMP00000033816
    • OTTHUMP00000033817
    • OTTHUMP00000038574
    • Protein tyrosine phosphatase receptor type c polypeptide
    • protein tyrosine phosphatase, receptor type, C
    • Ptprc
    • PTPRC_HUMAN
    • Receptor-type tyrosine-protein phosphatase C
    • T200
    • T200 glycoprotein
    • T200 leukocyte common antigen
    see all
  • 機能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 関連疾患Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • ドメインThe first PTPase domain interacts with SKAP1.
  • 翻訳後修飾Heavily N- and O-glycosylated.
  • 細胞内局在Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

Recombinant human CD45 protein (ab42584) 使用論文

ab42584 has not yet been referenced specifically in any publications.

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