Recombinant Human CD299 protein (Fc Chimera) (ab83920)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号Q9H2X3
    • 生物種Human
    • 配列Theoretical Sequence: HGTELPSPPSKLQVSKVPSSLSQEQSEQDAIYQNLTQLKAAVGELSEK SKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQ EIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQ ELTELKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTD LKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVI KTAEEQNFLQLQTSRSNRFSWMGLSDLNQEGTWQWVDGSPLSPSFQRYWN SGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACFRDGIPKVDKK VEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWL NGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • 配列の追加情報Fused with the Fc region of Human IgG1 at the C-terminus.

特性

Our Abpromise guarantee covers the use of ab83920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • C type lectin domain family 4, member M
    • C-type lectin domain family 4 member M
    • CD209 antigen like
    • CD209 antigen like protein 1
    • CD209 antigen-like protein 1
    • CD209L
    • CD209L1
    • CD299
    • CD299 antigen
    • CLC4M_HUMAN
    • CLEC4M
    • DC SIGN related protein
    • DC SIGN2
    • DC SIGNR
    • DC-SIGN-related protein
    • DC-SIGN2
    • DC-SIGNR
    • DCSIGN related protein
    • DCSIGNR
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 2
    • HP10347
    • L SIGN
    • L-SIGN
    • Liver/lymph node specific ICAM3 grabbing nonintegrin
    • Liver/lymph node-specific ICAM-3-grabbing non-integrin
    • LSIGN
    • Mannose binding C type lectin DC SIGNR
    • MGC129964
    • MGC47866
    see all
  • 機能Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
  • 組織特異性Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
  • 配列類似性Contains 1 C-type lectin domain.
  • ドメインThe tandem repeat domain, also called neck domain, mediates oligomerization.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt

Recombinant Human CD299 protein (Fc Chimera) 画像

  • Lane 1 – MW markers; Lane 2 – ab83920; Lane 3 – ab83920 treated with PNGase F to remove potential N-linked glycans. 10 μg protein loaded per lane. Deep Purple stained.
    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F protein.
  • A sample of ab83920 without carrier protein was reduced and alkylated. 40 μg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of ab83920. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as CD299 (Fc Chimera).
  • Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
    The triangle indicates theoretical pI and MW.

Recombinant Human CD299 protein (Fc Chimera) (ab83920) 使用論文

ab83920 has not yet been referenced specifically in any publications.

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