製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P55212
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab52157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性SPECIFIC ACTIVITY: 13,000 units/mg
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考

    UNIT DEFINITION: One unit of the recombinant Caspase-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: PBS, 15% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –80°C.

関連情報

  • 別名
    • Apoptotic protease Mch-2
    • Apoptotic protease MCH2
    • CASP-6
    • CASP6
    • CASP6_HUMAN
    • Caspase 6
    • Caspase 6 apoptosis related cysteine protease
    • Caspase 6, apoptosis related cysteine peptidase
    • Caspase-6 subunit p11
    • Mch2
    • OTTHUMP00000162742
    • OTTHUMP00000162743
    see all
  • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • 配列類似性Belongs to the peptidase C14A family.
  • 翻訳後修飾Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant human Caspase-6 protein (ab52157) 使用論文

This product has been referenced in:
  • Zandy AJ  et al. Role of the executioner caspases during lens development. J Biol Chem 280:30263-72 (2005). Read more (PubMed: 15994297) »
  • Basu A  et al. Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals. J Biol Chem 277:41850-6 (2002). Read more (PubMed: 12198125) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that the Caspase 6 protein (Active) (ab52157) is a tetramer with subunits 18kDa and 11kDa. On the reducing gel, you will see two bands.

I hope this information is helpful to you. Please do no...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"