Recombinant human CaMKII delta protein (ab84552)

製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      82 kDa including tags
    • 領域
      1 to 499
    • タグ
      GST tag N-Terminus

関連製品

特性

Our Abpromise guarantee covers the use of ab84552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The specific activity of ab84552 was determined to be 5860 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

    Other

  • 製品の状態
    Liquid
  • 備考

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Calcium / calmodulin dependent protein kinase 2 delta
    • Calcium / calmodulin dependent protein kinase II delta
    • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
    • calcium/calmodulin-dependent protein kinase type II delta chain
    • Calcium/calmodulin-dependent protein kinase type II subunit delta
    • CAM kinase 2 delta
    • CAM kinase II delta
    • CaM kinase II delta subunit
    • CaM kinase II subunit delta
    • CaM-kinase II delta chain
    • CAMK 2d
    • CaMK-II delta subunit
    • CaMK-II subunit delta
    • CAMK2D
    • CAMKD
    • CAMKI
    • KCC2D_HUMAN
    • RATCAMKI
    see all
  • 機能
    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
  • 組織特異性
    Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

画像

  • Kinase Assay. Specific activity 5860 nmol/min/mg.
  • SDS-PAGE of ab84552. Approximate MWt 77 kDa.

プロトコール

参考文献

ab84552 has not yet been referenced specifically in any publications.

レビューと Q&A

The substrate used with (ab84552) Active human CaMKII delta full length protein is the synthetic peptide KKALRRQETVDAL-amide. The peptide is diluted in distilled water to 1mg/ml. Normally use around 10ul of diluted kinase for a single kinase assay.

The recombinant protein also includes an N-terminal tag which has a molecular weight of 26kDa. This is the reason we expect to see ab84552 to produce a band near 72kDa. Thanks!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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