Recombinant Human Caldesmon protein (ab114302)

製品の概要

  • 製品名Recombinant Human Caldesmon protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号Q05682-4
    • 生物種Human
    • 分子量85 kDa including tags
    • 領域1 to 538

特性

Our Abpromise guarantee covers the use of ab114302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    ELISA

    SDS-PAGE

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • CAD
    • CALD 1
    • CALD1
    • CALD1_HUMAN
    • Caldesmon
    • Caldesmon 1
    • Caldesmon 1 Isoform 1
    • Caldesmon 1 Isoform 2
    • Caldesmon 1 Isoform 3
    • Caldesmon 1 Isoform 4
    • Caldesmon 1 Isoform 5
    • Caldesmon1
    • CDM
    • H CAD
    • HCAD
    • L CAD
    • LCAD
    • MGC21352
    • NAG22
    see all
  • 機能Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
  • 組織特異性High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
  • 配列類似性Belongs to the caldesmon family.
  • ドメインThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
  • 翻訳後修飾In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt

Recombinant Human Caldesmon protein 画像

  • 12.5% SDS-PAGE image showing ab114302 Stained with Coomassie Blue.

Recombinant Human Caldesmon protein (ab114302) 使用論文

ab114302 has not yet been referenced specifically in any publications.

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Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

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