Recombinant human BRG1 protein (ab82237)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab82237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性ab82237 contains no detectable proteases, DNase and RNase activity.
  • アプリケーション

    Functional Studies

    Western blot

  • 精製度> 95 % SDS-PAGE.
    ab82237 is greater than 95% homogeneous, purified by affinity and FPLC chromatography.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • ATP dependent helicase SMARCA4
    • ATP-dependent helicase SMARCA4
    • BAF 190
    • BAF190
    • BAF190A
    • Brahma protein like 1
    • BRG1
    • BRG1 associated factor 190A
    • BRG1 protein
    • BRG1-associated factor 190A
    • BRM/SWI2 related gene 1
    • Global transcription activator homologous sequence
    • global transcription activator snf2l4
    • Homeotic gene regulator
    • hSNF2b
    • Mitotic growth and transcription activator
    • MRD16
    • Nuclear protein GRB1
    • Protein brahma homolog 1
    • Protein BRG-1
    • Protein BRG1
    • RTPS2
    • SMARC A4
    • SMARCA4
    • SMCA4_HUMAN
    • SNF2
    • SNF2 beta
    • SNF2 like 4
    • SNF2-beta
    • SNF2B
    • SNF2L4
    • SNF2LB
    • Sucrose nonfermenting like 4
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
    • SWI2
    • Transcription activator BRG1
    see all
  • 機能Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
  • 組織特異性Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
  • 関連疾患Defects in SMARCA4 are the cause of rhabdoid tumor predisposition syndrome type 2 (RTPS2) [MIM:613325]. RTPS2 is a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood.
  • 配列類似性Belongs to the SNF2/RAD54 helicase family.
    Contains 1 bromo domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HSA domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt

Recombinant human BRG1 protein (ab82237) 使用論文

ab82237 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
I have to confirm that the delivery time for ab82237 is just under two weeks at the moment.
If you order ab82237 today you will receive it on Tuesday 19th of June.
I apologize for any inconvenience causes for yo...

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I can confirm that this protein is not an in-house product. However, we cannot name our suppliers as this is considered confidential information.

Thank you for your inquiry.
1. I am happy to confirm that Sf21 insect cells were used to produce ab82237.
2. ab82237 contains a 6x Histag at the N terminus and it is retained in the purified protein.
3. The protein will be soluble in Buf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"