The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
% SDS-PAGE. Greater than 98% by SDS-PAGE.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge the vial prior to opening. Reconstitute in water containing BSA (50ug of BSA per 1ug of protein) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Bone morphogenetic protein 2
Bone morphogenetic protein 2A
Induces cartilage and bone formation.
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.