Recombinant Human beta Tubulin protein (ab70187)

製品の概要

  • 製品名Recombinant Human beta Tubulin protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 分子量76 kDa including tags
    • 領域1 to 444
    • タグGST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab70187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.036% PMSF, 0.089% DTT, 17.819% Tris HCl, 25% Glycerol, 53.457% Sodium chloride, 3.564% Glutathione, 0.036% EDTA

関連情報

  • 別名
    • Beta 4 tubulin
    • Beta 5 tubulin
    • beta Ib tubulin
    • Beta1 tubulin
    • Class I beta tubulin
    • M40
    • MGC117247
    • MGC16435
    • OK/SW cl.56
    • OK/SWcl.56
    • TBB5_HUMAN
    • TUBB
    • TUBB 1
    • TUBB 2
    • TUBB 5
    • TUBB1
    • TUBB2
    • TUBB5
    • tubulin beta 1 chain
    • Tubulin beta 2 chain
    • tubulin beta 5 chain
    • Tubulin beta chain
    • Tubulin beta class I
    • tubulin beta polypeptide
    • Tubulin beta-5 chain
    see all
  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 組織特異性Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 関連疾患Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • 配列類似性Belongs to the tubulin family.
  • ドメインThe highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • 細胞内局在Cytoplasm, cytoskeleton.
  • Information by UniProt

Recombinant Human beta Tubulin protein 画像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant Human beta Tubulin protein (ab70187) 使用論文

ab70187 has not yet been referenced specifically in any publications.

Product Wall

ab70187 contains a N-terminal GST tag, which is 26 kDa.



We also have an extensive range of beta tubulin antibodies if you are looking for one through the link below:



http://www.abcam.com/products?keywords=beta+...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"