Recombinant Human beta Tubulin protein (ab70187)
Key features and details
- Expression system: Escherichia coli
- Purity: > 70% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
製品の詳細
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製品名
Recombinant Human beta Tubulin protein -
精製度
> 70 % Densitometry.
Purity is lot specific. Please contact our technical Support team for details. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
76 kDa including tags -
領域
1 to 444 -
タグ
GST tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab70187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA
関連情報
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別名
- Beta 4 tubulin
- Beta 5 tubulin
- beta Ib tubulin
see all -
機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
組織特異性
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
関連疾患
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70187 は 3 報の論文で使用されています。
- Berke NA et al. Critical Sites on Ostreolysin Are Responsible for Interaction with Cytoskeletal Proteins. Biomedicines 10:N/A (2022). PubMed: 36289704
- Sharafeldin M et al. Detecting cancer metastasis and accompanying protein biomarkers at single cell levels using a 3D-printed microfluidic immunoarray. Biosens Bioelectron 171:112681 (2021). PubMed: 33096435
- Wei J et al. Anti-proliferative effect of isorhamnetin on HeLa cells through inducing G2/M cell cycle arrest. Exp Ther Med 15:3917-3923 (2018). PubMed: 29563987